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This is an important factor when considering the phylogenetic conservation of these Canadian equine populations. Different molecular tools and techniques have been utilized to assess the origin and genetic diversity present within a species, including mitochondrial DNA (mt DNA) sequence data (Vilà et al. Sequencing reactions were performed using Big Dye Terminator Cycle Sequencing Kits v.

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Within the Canadian populations, haplotype diversity was highest in the Newfoundland pony (0.96 ± 0.08), whereas pairwise difference and nucleotide diversity was highest in the Canadian horse (7.090 ± 3.581 and 0.0188 ± 0.0042, respectively). PCR protocols involved an initial denaturation at 94 °C for 2 min followed by 30 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min.

Three different estimates of genetic distances were used to examine the phylogenetic relationships amongst these populations. In general, the native Canadian populations were highly represented in the D clade, with particular emphasis in the D1 and D2 clades. PCR amplicons were purified using Exonuclease-I and Shrimp Alkaline Phosphatase (Fermentas Canada Inc., Burling, Ontario) according to the manufacturer's specifications.

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Reveille VIII, a sable and white AKC-registered collie from Topeka, Kan., will retire at the end of the 2014-15 academic year.

The inclusion of 10 Mountain and Moorland, 3 Nordic pony breeds, 2 feral populations, and 5 horse breeds were also investigated as they may have influenced the development (or rejuvenation) of the native Canadian populations. This study examines the genetic diversity and relationships among the native Canadian equine populations and their relationship to the Mountain and Moorland (Connemara, Dale, Dartmoor, Eriskay, Exmoor, Fell, Highland, Kerry Bog, New Forest, Shetland, and Welsh) and Nordic (Fjord, Icelandic, and Shetland) pony breeds using a 378-bp section of the mt DNA D-loop. In addition to samples collected from the native Canadian equine populations (Canadian, Lac La Croix, Newfoundland, and Sable Island), samples were also collected from the Mountain and Moorland (Connemara, Dale, Dartmoor, Eriskay, Exmoor, Fell, Highland, Kerry Bog, New Forest, and Welsh), Nordic (Fjord, Icelandic, and Shetland), and historically important horse and feral populations.

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